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ARGEN graph reference

Data & methods.

Source conditions, trace labels and accessible values for the graph animations.

Illustrative simulation

Four fast, two medium, two stable.

Illustrative 24-hour study | eight parallel samples | Mw(t)/M0

Illustrative simulation. Not measured data. Eight synthetic trajectories show four fast-aggregating, two medium and two stable samples. Time runs from 0 to 24 hours; the y-axis is normalized weight-average molecular weight, Mw(t)/M0, where M0 is the initial value. Playback shows the study in 12 seconds, followed by a two-second hold. The sample cells show schematic aggregation, not molecular simulation or imaging.

Illustrative endpoints, not experimental results. All eight samples begin at Mw/M0 = 1.
SampleIllustrative responseInitial Mw/M0Mw/M0 at 24 h
S1Fast1.004.60
S2Fast1.004.10
S3Fast1.003.65
S4Fast1.003.20
S5Medium1.002.30
S6Medium1.001.90
S7Stable1.001.00
S8Stable1.001.03

Source chart reconstruction

Concentration under stirring stress

BSA | 25 °C | 500 rpm | 12 h

Approximate reconstruction from the supplied chart. Not raw measurement data. Linear time axis in seconds, from 0 to 43,200 (12 hours). Eight source-labeled concentrations are shown under the reported stirring conditions. The y-axis shows normalized weight-average molecular weight, Mw(t)/M0, where M0 is the initial weight-average molecular weight. Playback compresses time into 12 seconds, followed by a two-second hold. Curves stop at the source bounds.

Approximate last visible values reconstructed from the chart; original raw values are unavailable.
TraceSource conditionLast visible timeNormalized Mw
A0.5 mg/mL4.29e+04 s~1.14
B1.0 mg/mL4.29e+04 s~1.72
C1.5 mg/mL4.29e+04 s~2.1
D2.0 mg/mL4.29e+04 s~2.78
E4.0 mg/mL4.29e+04 s~7.38
F6.0 mg/mL4.29e+04 s~11.1
G8.0 mg/mL4.29e+04 s~4.27
H10 mg/mL4.29e+04 s~8.17

Source chart reconstruction

Temperature-dependent aggregation

mAb | 1 mg/mL | source range 50-80 °C | 8 selected traces

Eight selected source traces reconstructed from the supplied chart. Not raw measurement data. Logarithmic time axis in seconds, from 10 to 50,400. Eight selected source traces are shown: the labeled 80 °C and 50 °C conditions plus six intermediate curves whose temperatures are not identified in the source. This is a selected view of the source chart, not a new eight-cell experiment. The y-axis shows normalized weight-average molecular weight, Mw(t)/M0, where M0 is the initial weight-average molecular weight. Playback compresses time into 12 seconds, followed by a two-second hold. Curves stop at the source bounds.

Approximate last visible values reconstructed from the chart; original raw values are unavailable.
TraceSource conditionLast visible timeNormalized Mw
A80 °C48 s~11.9
BTemperature unlabelled63.8 s~11.9
CTemperature unlabelled135 s~11.9
DTemperature unlabelled412 s~11.9
ETemperature unlabelled1.32e+03 s~11.9
FTemperature unlabelled7.75e+03 s~11.9
GTemperature unlabelled4.57e+04 s~10.6
H50 °C4.84e+04 s~1.81

Source chart reconstruction

pH-dependent molecular-weight change

BSA | 55 °C | logarithmic time in hours

Approximate reconstruction from the supplied chart. Not raw measurement data. Logarithmic time axis in hours, from 0.01 to 100. The three source-labeled pH conditions and source-reported rate annotations are retained; no new rate model has been fitted. The y-axis shows normalized weight-average molecular weight, Mw(t)/M0, where M0 is the initial weight-average molecular weight. Playback compresses time into 12 seconds, followed by a two-second hold. Curves stop at the source bounds.

Approximate last visible values reconstructed from the chart; original raw values are unavailable.
TraceSource conditionLast visible timeNormalized Mw
ApH 4.750.0679 h~3.98
BpH 6.7546.6 h~2.24
CpH 2.7595.6 h~0.622